ELISA: 0.5-1.0 µg/mL, IP: 25 µg/mL, WB: 1-2 µg/mL Optimal conditions should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 0.5 mg/mL.
浓度
Lot specific
缓冲液
Supplied lyophilized from a 0.2 µm filtered solution in PBS, with 5 % Trehalose.
注意事项
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
Carboxypeptidase E (CPE) is a peripheral membrane protein that cleaves C-terminal amino acid residues and is involved in the processing of numerous peptide hormones and neurotransmitters. CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance. The CPE precursor includes a signal peptide (aa 1 - 25), a propeptide (aa 26 - 42) and a mature chain (aa 43 - 476). The amino acid sequence of human CPE is 99.8%, 96.6%, 96.3%, and 90.2% identical to that of chimpanzee, mouse, dog and chicken. Synonyms: Monoclonal Carboxypeptidase E antibody, Anti-Carboxypeptidase E antibody.