T7-Tag 抗体
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- 抗原 See all T7-Tag products
- T7-Tag
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This T7-Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 序列
- MASMTGGQQM G
- 纯化方法
- Affinity purified
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anti-T7-Tag antibody
WB 宿主: 小鼠 Monoclonal 1M4 unconjugated
anti-T7-Tag antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-T7-Tag antibodyWB 宿主: 小鼠 Monoclonal 1H5 unconjugated
anti-T7-Tag antibodyWB, IP 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- Western blot: Use at 1:5,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.2 and 0.1% sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- T7-Tag
- 别名
- T7 tag (T7-Tag 产品)
- 物质类
- Tag
- 背景
- Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support. Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. The anti-T7 diluted 1:35,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier than was used for purification. HRP-conjugated donkey anti-rabbit IgG was used and TMB was the substrate.
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