Nck1/Nck alpha (AA 31-150) 抗体 (Alexa Fluor 647)
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- 抗原
- Nck1/Nck alpha
- 抗原表位
- AA 31-150
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Alexa Fluor 647
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应用范围
- Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- 预测反应
- Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human Nck2
- 亚型
- IgG
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anti-Nck1/Nck alpha (AA 31-150) antibody
适用: 人 IF (cc), IF (p), IHC (p), ELISA, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Nck1/Nck alpha
- 背景
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Synonyms: NCK adaptor protein 2, Cytoplasmic protein NCK1, Melanoma Nck protein, MGC12668, NCK 1, Nck adaptor protein 1, NCK tyrosine kinase, NCK1, NCKalpha, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK alpha.
Background: Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.
- 基因ID
- 4690
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