Rhodopsin 抗体
(2 references)Quick Overview for Rhodopsin 抗体 (ABIN863085)
抗原
See all Rhodopsin (RHO) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Detects ~40 kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
-
交叉反应
- Vertebrate
-
纯化方法
- Protein G Purified
-
免疫原
- Bovine Rhodopsin
-
亚型
- IgG1
-
-
anti-Rhodopsin (RHO) antibody (HRP)
RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 HRP
anti-Rhodopsin (RHO) antibody (FITC)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 FITC
anti-Rhodopsin (RHO) antibody (Biotin)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Biotin
anti-Rhodopsin (RHO) antibody (PE)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 PE
anti-Rhodopsin (RHO) antibody (PerCP)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 PerCP
anti-Rhodopsin (RHO) antibody (APC)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 APC
anti-Rhodopsin (RHO) antibody (Atto 488)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 488
anti-Rhodopsin (RHO) antibody (Atto 594)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 594
anti-Rhodopsin (RHO) antibody (Atto 390)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 390
anti-Rhodopsin (RHO) (Extracellular Domain) antibodyRHO 适用: 人, 大鼠, 小鼠, Pig, 猴, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
- WB (1:1000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
-
说明
-
1 μg/ml of ABIN863084 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- -20°C
-
-
-
: "Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision." in: Nature communications, Vol. 10, Issue 1, pp. 1281, (2019) (PubMed).
: "Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy." in: Methods in molecular biology (Clifton, N.J.), Vol. 935, pp. 185-99, (2012) (PubMed).
-
: "Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision." in: Nature communications, Vol. 10, Issue 1, pp. 1281, (2019) (PubMed).
-
- Rhodopsin (RHO)
-
别名
- Rhodopsin
-
物质类
- Chemical
-
背景
- Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
-
基因ID
- 509933
-
NCBI登录号
- NP_001014890
-
UniProt
- P02699
-
途径
- WNT signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
抗原
-
