Rhodopsin 抗体 (Extracellular Domain)
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- 抗原 See all Rhodopsin (RHO) 抗体
- Rhodopsin (RHO)
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抗原表位
- Extracellular Domain
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适用
- 人, 大鼠, 小鼠, Pig, 猴, 兔
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Rhodopsin antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human RHO / Rhodopsin. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from 2nd extracellular domain of human RHO / Rhodopsin. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product RHO Primary Antibody
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anti-Rhodopsin (RHO) antibody
RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Rhodopsin (RHO) antibody (HRP)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 HRP
anti-Rhodopsin (RHO) antibody (FITC)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 FITC
anti-Rhodopsin (RHO) antibody (Alkaline Phosphatase (AP))RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Alkaline Phosphatase (AP)
anti-Rhodopsin (RHO) antibody (Biotin)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Biotin
anti-Rhodopsin (RHO) antibody (PE)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 PE
anti-Rhodopsin (RHO) antibody (APC)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 APC
anti-Rhodopsin (RHO) antibody (PerCP)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 PerCP
anti-Rhodopsin (RHO) antibody (Atto 488)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 488
anti-Rhodopsin (RHO) antibody (Atto 594)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 594
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- 应用备注
- Approved: IHC, IHC-P (1 - 3 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Rhodopsin (RHO)
- 别名
- Rhodopsin / rho (RHO 产品)
- 别名
- CSNBAD1 antibody, OPN2 antibody, RP4 antibody, RH1 antibody, Rh antibody, fi06d11 antibody, wu:fi06d11 antibody, zfo2 antibody, zfrho antibody, Noerg1 antibody, Opn2 antibody, Ops antibody, opn2 antibody, rh1 antibody, rp4 antibody, xrho antibody, PIGRHO1 antibody, ops antibody, RDP1 antibody, RHO antibody, rhodopsin antibody, rhodopsin L homeolog antibody, RHO antibody, Rho antibody, rho antibody, rho.L antibody
- 物质类
- Chemical
- 背景
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Name/Gene ID: RHO
Subfamily: Opsin
Family: GPCR
Synonyms: RHO, CSNBAD1, OPN2, Rhodopsin, RP4, Opsin 2, rod pigment, Opsin-2 - 基因ID
- 6010
- 途径
- WNT signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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