Neuron Specific beta-Tubulin III (C-Term) 抗体
适用: 人, 小鼠, 大鼠
WB
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Neuron Specific beta-Tubulin III
- 抗原表位
- C-Term
- 适用
- 人, 小鼠, 大鼠
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 特异性
- Neuron Specific beta-Tubulin III Antibody, pAb, Rabbit detects endogenous levels of human, mouse, and rat neuron specific beta-Tubulin III and does not cross-react with other beta tubulin isotypes. Positive Control: Mouse brain, rat brain, SY-SH5H and U251.
- 交叉反应 （详细）
- Neuron Specific beta-Tubulin III Antibody, pAb, Rabbit detects endogenous levels of human, mouse, and rat neuron specific beta-Tubulin III and does not cross-react with other beta tubulin isotypes.Positive Control: Mouse brain, rat brain, SY-SH5H and U251.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- KLH-coupled synthetic peptide from C-terminal of human neuron specific β-Tubulin III
- 亚型
- IgG
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mL Other Applications: user optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- 抗原
- Neuron Specific beta-Tubulin III
- 背景
- Neuron specific beta-Tubulin III, also known as tubulin beta-4, belongs to the tubulin family. It is the major constituent of microtubules and forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one binding is an exchangeable site of the beta chain and the other is at a non exchangeable site of the alpha chain. Neuron specific beta-Tubulin III is regarded as a neuron-specific marker. The expression of neuron specific beta-Tubulin III has been implicated in signal neuronal commitment in primitive neuroepithelium. In addition, increased expression of neuron specific beta-Tubulin III occurs during cancer formation which leads to the development of anti-tubulin drugs. Neuron Specific beta-Tubulin III Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human neuron specific beta-Tubulin III (Swiss Prot: Q13509). This antibody label neuronal cell bodies, dendrites, axons, and axonal terminations and is used identify newly committed neurons. In addition, it serves an excellent loading control when normalizing data specifically for neuronal expression.
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