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SPARC 抗体 (AA 107-303)

The 兔 多克隆 anti-SPARC antibody (ABIN8035174) specifically detects SPARC in WB. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN8035174
发货至: 中国
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Room 801-803
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Quick Overview for SPARC 抗体 (AA 107-303) (ABIN8035174)

抗原

See all SPARC 抗体
SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

适用

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人, 小鼠, 大鼠

宿主

  • 140
  • 56
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克隆类型

  • 136
  • 64
  • 5
多克隆

标记

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This SPARC antibody is un-conjugated

应用范围

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Western Blotting (WB)

质量等级

Carrier-free
  • 抗原表位

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    • 2
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    • 1
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    AA 107-303

    原理

    Anti-SPARC Antibody

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    预测反应

    Human SPARC shares 97% amino acid (aa) sequence identity with both mouse,rat SPARC.

    产品特性

    Anti-SPARC Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

    别名

    SPARC

    背景

    Background: SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.

    Gene Full Name: secreted protein acidic and cysteine rich

    分子量

    35 kDa

    基因ID

    6678

    UniProt

    P09486

    途径

    Autophagy
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