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SPARC 抗体 (AA 107-303) (HRP)

The 兔 多克隆 anti-SPARC antibody is suitable to detect SPARC in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IHC 和 ELISA.
产品编号 ABIN8035170
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for SPARC 抗体 (AA 107-303) (HRP) (ABIN8035170)

抗原

See all SPARC 抗体
SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This SPARC antibody is conjugated to HRP

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • 抗原表位

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    AA 107-303

    原理

    Anti-SPARC Antibody HRP Conjugated

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    预测反应

    Human SPARC shares 97% amino acid (aa) sequence identity with both mouse,rat SPARC.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.

    亚型

    IgG
  • 应用备注

    Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

    储存条件

    -20 °C

    储存方法

    At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

    有效期

    12 months
  • 抗原

    SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

    别名

    SPARC

    背景

    Background: SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.

    Gene Full Name: secreted protein acidic and cysteine rich

    基因ID

    6678

    UniProt

    P09486

    途径

    Autophagy
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