PRKCA beta 2 抗体 (pThr638, pThr641)
(3 references)-
- 抗原 See all PRKCA beta 2 products
- PRKCA beta 2 (Protein Kinase C alpha beta 2 (PRKCA beta 2))
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抗原表位
- pThr638, pThr641
- 适用
- 人, 小鼠, 大鼠, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRKCA beta 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 犬, 小鼠, 大鼠
- 预测反应
- Human,Cow,Horse,Chicken
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic phosphopeptide derived from human PKC alpha/beta II around the phosphorylation site of Thr638/641
- 亚型
- IgG
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anti-Protein Kinase C alpha beta 2 (PRKCA beta 2) (pThr638), (pThr641) antibody
PRKCA beta 2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells." in: Molecular biology of the cell, Vol. 28, Issue 10, pp. 1389-1399, (2017) (PubMed).
: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).
: "cPKC regulates interphase nuclear size during Xenopus development." in: The Journal of cell biology, Vol. 206, Issue 4, pp. 473-83, (2014) (PubMed).
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: "PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells." in: Molecular biology of the cell, Vol. 28, Issue 10, pp. 1389-1399, (2017) (PubMed).
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- 抗原
- PRKCA beta 2 (Protein Kinase C alpha beta 2 (PRKCA beta 2))
- Abstract
- PRKCA beta 2 产品
- 背景
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Synonyms: AAG6, PKCA, PRKACA, PKC-alpha, Protein kinase C alpha type, PKC-A, PRKCA
Background: Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.
- 基因ID
- 5578
- UniProt
- P17252
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