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G-CSF 抗体

This 小鼠 单克隆 antibody specifically detects G-CSF in IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN7880489
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for G-CSF 抗体 (ABIN7880489)

抗原

See all G-CSF (CSF3) 抗体
G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This G-CSF antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM468
  • 原理

    GCSF Antibody

    纯化方法

    Protein G affinity chromatography

    免疫原

    Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes was used as the immunogen for the GCSF antibody.

    亚型

    IgG1, kappa
  • 应用备注

    Optimal dilution of the GCSF antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the GCSF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

    别名

    GCSF

    背景

    This mAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSFs, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73 % sequence identity at the amino acid level.

    UniProt

    P09919

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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