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G-CSF 抗体

CSF3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465577
发货至: 中国
  • 抗原 See all G-CSF (CSF3) 抗体
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    适用
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    宿主
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    多克隆
    标记
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    This G-CSF antibody is un-conjugated
    应用范围
    • 74
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Human G-CSF
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human G-CSF.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product CSF3 Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hG-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. ELISA: To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (LS-C104674) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute with sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    别名
    CSF3 / G-CSF / GCSF (CSF3 产品)
    别名
    CSF3 antibody, G-CSF antibody, gcsf antibody, Csfg antibody, MGI-IG antibody, C17orf33 antibody, CSF3OS antibody, GCSF antibody, Gcsf antibody, colony stimulating factor 3 antibody, colony stimulating factor 3 (granulocyte) a antibody, colony stimulating factor 3 (granulocyte) antibody, CSF3 antibody, csf3a antibody, Csf3 antibody
    背景
    Name/Gene ID: CSF3

    Synonyms: CSF3, C17orf33, Filgrastim, G-CSF, GCSF, Pluripoietin, CSF3OS, Lenograstim
    基因ID
    1440
    UniProt
    P09919
    途径
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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