Recombinant PRKACA 抗体 (pThr197)
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北京 101111
Quick Overview for Recombinant PRKACA 抗体 (pThr197) (ABIN7879786)
抗原
See all PRKACA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pThr197
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原理
- Phospho-PKA C (Thr197) Antibody / PRKACA/PRKACB/PRKACG
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Phospho-PKA C (Thr197) was used as the immunogen for the Phospho-PKA C (Thr197) antibody.
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亚型
- IgG
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anti-Protein Kinase A, alpha (PRKACA) (Center) antibody
PRKACA 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibodyPRKACA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-Protein Kinase A, alpha (PRKACA) (Internal Region) antibodyPRKACA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (pThr197) antibodyPRKACA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 233-351), (Catalytic Subunit) antibodyPRKACA 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibodyPRKACA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H3A4 unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 67-94), (N-Term) antibodyPRKACA 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20762 unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibodyPRKACA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (N-Term) antibodyPRKACA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (Thr198) antibodyPRKACA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Phospho-PKA C (Thr197) antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-PKA C (Thr197) antibody at -20oC.
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- PRKACA (Protein Kinase A, alpha (PRKACA))
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别名
- PKA C
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背景
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Phospho-PKA C (Thr197) antibody detects Protein kinase A catalytic subunit phosphorylated at threonine 197, encoded by PRKACA, PRKACB, and PRKACG. Protein kinase A catalytic subunit is the enzymatic component of the cAMP-dependent protein kinase holoenzyme, which regulates metabolism, growth, and gene expression. Phosphorylation at threonine 197 is required for full activation of the catalytic subunit. Phospho-PKA C (Thr197) antibody provides researchers with a highly specific reagent to study activation of PKA signaling in multiple biological systems.
Protein kinase A catalytic subunit is activated when cAMP binds regulatory subunits, releasing the catalytic subunits into the cytoplasm. Research using Phospho-PKA C (Thr197) antibody has demonstrated that phosphorylation at threonine 197 occurs within the activation loop and is necessary for stabilizing the active conformation. This phosphorylation is typically mediated by phosphoinositide-dependent kinase 1 and ensures robust catalytic activity toward downstream substrates. Without this modification, kinase activity is impaired, highlighting its essential role in PKA signaling.
Studies with Phospho-PKA C (Thr197) antibody have revealed that phosphorylation of the catalytic subunit regulates diverse cellular processes. These include glycogen metabolism, lipolysis, ion channel regulation, and transcriptional activation. PKA phosphorylates transcription factors such as CREB, influencing gene expression programs in response to hormonal and metabolic signals. By monitoring phosphorylation at Thr197, researchers gain insight into dynamic activation of cAMP-dependent signaling.
Dysregulation of PKA phosphorylation has been linked to disease. Research using Phospho-PKA C (Thr197) antibody has shown that abnormal activity contributes to endocrine disorders, cardiovascular disease, and cancer. Mutations in PRKACA are associated with adrenal hyperplasia and Cushing's syndrome, conditions characterized by excessive PKA signaling. In oncology, altered phosphorylation of PKA subunits affects cell proliferation and survival, supporting tumor growth. These findings emphasize the importance of Thr197 phosphorylation in physiology and pathology.
Phospho-PKA C (Thr197) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes active phosphorylated subunits, immunohistochemistry highlights activated kinase in tissues, and immunofluorescence demonstrates localization of phosphorylated subunits in response to stimuli. These applications make Phospho-PKA C (Thr197) antibody an indispensable tool in signaling research.
By supplying validated Phospho-PKA C (Thr197) antibody reagents, NSJ Bioreagents supports studies into kinase signaling, endocrine biology, and cancer. Detection of phosphorylation at threonine 197 in PRKACA, PRKACB, and PRKACG provides a precise marker of Protein kinase A catalytic activity. -
UniProt
- P22612
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途径
- NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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