PSME4 抗体 (AA 391-657)
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Quick Overview for PSME4 抗体 (AA 391-657) (ABIN7874615)
抗原
See all PSME4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 391-657
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原理
- PSME4 Antibody / Proteasome activator complex subunit 4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PSME4 recombinant protein (Position: Q391-L657) was used as the immunogen for the PSME4 antibody.
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亚型
- IgG
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anti-Proteasome (Prosome, Macropain) Activator Subunit 4 (PSME4) (AA 1634-1843) antibody
PSME4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Proteasome (Prosome, Macropain) Activator Subunit 4 (PSME4) (AA 105-198) antibodyPSME4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Proteasome (Prosome, Macropain) Activator Subunit 4 (PSME4) (AA 1-50) antibodyPSME4 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Proteasome (Prosome, Macropain) Activator Subunit 4 (PSME4) (AA 503-535) antibodyPSME4 适用: 人 WB 宿主: 兔 Polyclonal RB55279 unconjugated
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应用备注
- Optimal dilution of the PSME4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PSME4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PSME4 (Proteasome (Prosome, Macropain) Activator Subunit 4 (PSME4))
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别名
- PSME4
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背景
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PSME4 antibody detects Proteasome activator complex subunit 4, also known as PA200, a nuclear protein that regulates proteasome function during DNA repair and spermatogenesis. PSME4 binds to the 20S proteasome core to enhance peptide cleavage and modulate substrate selectivity in a ubiquitin-independent manner. The PSME4 antibody is widely used in proteostasis, cell cycle, and DNA repair research to study proteasome regulation and nuclear protein degradation.
PSME4 is encoded by the PSME4 gene located on human chromosome 2q37.1. The protein is approximately 1,840 amino acids long and forms a ring-like structure that caps one end of the 20S proteasome. PSME4-mediated activation alters proteasome activity toward oxidized and acetylated substrates, enhancing degradation of histones and DNA repair factors following genotoxic stress.
The PSME4 antibody detects a 220 kilodalton protein by western blot and shows strong nuclear localization under immunofluorescence microscopy. PSME4 plays a crucial role in DNA double-strand break repair by facilitating turnover of repair-associated chromatin proteins, including histones and Ku70/80. In male germ cells, PSME4 regulates histone replacement during spermatogenesis, ensuring chromatin condensation and sperm maturation.
Deficiency of PSME4 leads to impaired proteasome activation and accumulation of damaged proteins, resulting in genomic instability and infertility. PSME4 also influences transcriptional regulation and aging by modulating histone acetylation and chromatin dynamics. In cancer, aberrant PSME4 expression alters proteasome activity, contributing to resistance against apoptosis and therapeutic stress.
As a specialized proteasome activator, PSME4 provides insight into the noncanonical regulation of proteolysis and DNA repair. NSJ Bioreagents provides a validated PSME4 antibody optimized for its applications, supporting research into nuclear proteasome regulation, DNA damage response, and reproductive biology. -
UniProt
- Q14997
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途径
- DNA Replication, Synthesis of DNA
抗原
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