IRAK4 抗体 (AA 15-460)
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北京 101111
Quick Overview for IRAK4 抗体 (AA 15-460) (ABIN7870794)
抗原
See all IRAK4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 15-460
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原理
- IRAK4 Antibody / Interleukin-1 receptor-associated kinase 4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human IRAK4 recombinant protein (Position: N15-S460) was used as the immunogen for the IRAK4 antibody.
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亚型
- IgG
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anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) antibody
IRAK4 适用: 人, 小鼠, 猴 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2H9 unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 189-427) antibodyIRAK4 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 1-220) antibodyIRAK4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 5-36), (N-Term) antibodyIRAK4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2347 unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 1-130) antibodyIRAK4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D3 unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (N-Term) antibodyVerified IRAK4 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (pThr345) antibodyIRAK4 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 1-130) antibodyIRAK4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (AA 25-52), (N-Term) antibodyIRAK4 适用: 人 WB 宿主: 兔 Polyclonal RB33094 unconjugated
anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (pThr345) antibodyIRAK4 适用: 人, 小鼠 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the IRAK4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the IRAK4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
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别名
- IRAK4
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背景
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IRAK4 antibody detects Interleukin-1 receptor-associated kinase 4, a serine/threonine kinase that mediates innate immune signaling through Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). Encoded by the IRAK4 gene on chromosome 12q12, this kinase functions as a central adaptor and activator in MyD88-dependent inflammatory pathways. Upon receptor activation, IRAK4 phosphorylates downstream kinases and recruits TRAF6, leading to activation of NF-kB and MAPK signaling cascades that drive cytokine production and immune defense.
Structurally, IRAK4 contains an N-terminal death domain that mediates interaction with MyD88 and IRAK1, a kinase domain responsible for autophosphorylation, and regulatory regions that ensure signal specificity. IRAK4 acts as the first kinase activated in the TLR/IL-1R pathway, enabling the assembly of the Myddosome complex. This signaling cascade is essential for early innate immune responses against bacterial and viral pathogens.
The IRAK4 antibody is widely used in immunology, inflammation, and infection research to study signal transduction, cytokine regulation, and kinase activation. Western blot analysis detects a 52 kilodalton band corresponding to IRAK4, while immunofluorescence and immunohistochemistry show cytoplasmic staining in immune and epithelial cells. This antibody supports characterization of TLR signaling and innate immune responses in both normal and pathological states.
Deficiency or loss-of-function mutations in IRAK4 cause primary immunodeficiency characterized by impaired IL-1 and TLR signaling and recurrent bacterial infections. Conversely, overactivation contributes to chronic inflammation, autoimmune diseases, and cancer. The IRAK4 antibody provides a reliable tool for exploring innate immune signaling and therapeutic kinase targeting. NSJ Bioreagents supplies this antibody validated for its applications to ensure consistent detection across immune research applications. -
UniProt
- Q9NWZ3
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途径
- NF-kappaB Signaling, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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