Axin 抗体 (AA 1-811)
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北京 101111
Quick Overview for Axin 抗体 (AA 1-811) (ABIN7869571)
抗原
See all Axin (AXIN1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-811
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原理
- AXIN1 Antibody / Axin-1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human AXIN1 recombinant protein (Position: M1-K811) was used as the immunogen for the AXIN1 antibody.
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亚型
- IgG
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anti-Axin 1 (AXIN1) (AA 546-752) antibody
AXIN1 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 4E9F1 unconjugated
anti-Axin 1 (AXIN1) (AA 618-827) antibodyAXIN1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Axin 1 (AXIN1) (AA 643-740) antibodyAXIN1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Axin 1 (AXIN1) (AA 546-752) antibodyAXIN1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4F10G1 unconjugated
anti-Axin 1 (AXIN1) (AA 653-862) antibodyAXIN1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Axin 1 (AXIN1) (AA 710-738), (C-Term) antibodyAXIN1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB31751 unconjugated
anti-Axin 1 (AXIN1) (AA 710-738) antibodyAXIN1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Axin 1 (AXIN1) (AA 761-862) antibodyAXIN1 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Axin 1 (AXIN1) (AA 654-863) antibodyAXIN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Axin 1 (AXIN1) (C-Term) antibodyVerified AXIN1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Optimal dilution of the AXIN1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the AXIN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Axin (AXIN1) (Axin 1 (AXIN1))
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别名
- AXIN1
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背景
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AXIN1 antibody detects Axin-1, encoded by the AXIN1 gene on chromosome 16p13.3. AXIN1 antibody is widely applied in studies of Wnt signaling, cell fate determination, and cancer. Axin-1 is a scaffold protein that functions as a negative regulator of the canonical Wnt signaling pathway by promoting degradation of beta-catenin. It is essential for development, stem cell maintenance, and oncogenesis regulation. Through its multiple interaction domains, Axin-1 coordinates formation of the beta-catenin destruction complex, ensuring tight regulation of Wnt target gene expression.
Structurally, Axin-1 is a ~96 kDa cytoplasmic protein composed of an RGS domain, coiled-coil regions, and interaction motifs for APC, GSK3, and beta-catenin. These domains enable assembly of the multiprotein destruction complex. Axin-1 also binds Dishevelled, tankyrase, and other regulators that modulate Wnt activity. Alternative isoforms exist with altered binding properties, influencing Wnt signaling output.
Functionally, Axin-1 acts as a central scaffold in beta-catenin regulation. In the absence of Wnt ligands, Axin-1 recruits beta-catenin, APC, CK1, and GSK3 to promote phosphorylation and degradation of beta-catenin, suppressing transcriptional activation. Upon Wnt stimulation, Axin-1 is destabilized by tankyrase-mediated poly(ADP-ribosyl)ation, allowing beta-catenin accumulation and target gene activation. Researchers use AXIN1 antibody to study Wnt pathway regulation, developmental processes, and tumorigenesis.
Clinically, AXIN1 mutations and deletions have been identified in hepatocellular carcinoma, medulloblastoma, and colorectal cancer, underscoring its tumor suppressor role. Dysregulation of Axin-1 contributes to abnormal Wnt signaling, a hallmark of many cancers. AXIN1 is also implicated in developmental defects and stem cell differentiation. Because Wnt signaling is a therapeutic target, Axin-1 is under investigation as a biomarker and potential drug target. NSJ Bioreagents supplies AXIN1 antibody for Wnt signaling, cancer, and developmental research.
Experimentally, AXIN1 antibody is applied in western blotting to detect the ~96 kDa protein, in immunofluorescence microscopy to assess cytoplasmic puncta, and in immunohistochemistry to study tumor tissue. Co-immunoprecipitation with AXIN1 antibody identifies destruction complex partners and signaling regulators. -
UniProt
- O15169
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途径
- WNT signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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