Recombinant KAP1 抗体 (Center)
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Quick Overview for Recombinant KAP1 抗体 (Center) (ABIN7826150)
抗原
See all KAP1 (TRIM28) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Center
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原理
- KAP1 / TRIM28 antibody (HMV335)
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免疫原
- Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A strong nuclear TRIM28are staining should be seen in all cells.
Product Description/Background: KAP1 / TRIM28 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Testis: Spermatids are TRIM28 negative while all other cells show a strong nuclear TRIM28 staining of all cells.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
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别名
- KAP1 / TRIM28
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背景
- Gene Alias: tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B
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UniProt
- Q13263
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途径
- Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
抗原
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