我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

LEF1 抗体 (AA 331-399)

Name: LEF1 抗体 (AA 331-399)
SKU: ABIN735248
Availability: OutOfStock

This anti-LEF1 antibody is a 兔多克隆 antibody detecting LEF1 in WB, ELISA 和 IHC (p). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

Mouse lymph node lysates probed with Rabbit Anti-LEF-1 Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C. (LEF1 抗体 (AA 331-399))

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-LEF-1 Polyclonal Antibody, Unconjugated (ABIN735248) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining (LEF1 抗体 (AA 331-399))

IHC

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-LEF-1 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (LEF1 抗体 (AA 331-399))

IHC (p)

L1 rat brain lysates L2 human colon carcinoma lysates probed with Anti IL-2R gamma/CD132 Polyclonal Antibody, Unconjugated (ABIN735248) at 1:200 overnight at 4 °C. (LEF1 抗体 (AA 331-399))

SDS

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-LEF-1 Polyclonal Antibody, Unconjugated (ABIN735248) at 1:200 followed by conjugation to the secondary antibody and DAB staining (LEF1 抗体 (AA 331-399))

IHC

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-LEF-1 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining (LEF1 抗体 (AA 331-399))

IHC (p)

6 images

(3 references)

产品编号 ABIN735248

发货至: 中国

Quick Overview for LEF1 抗体 (AA 331-399) (ABIN735248)

抗原

See all LEF1 抗体

LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This LEF1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product LEF1 Primary Antibody

抗原表位
9

7

6

5

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 331-399

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Pig,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human LEF-1

亚型

IgG
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody
LEF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 1-399) antibody
LEF1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3H5 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody
Verified LEF1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 33-138) antibody
LEF1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1A4B11 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 14-123) antibody
LEF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A3 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 75-216) antibody
LEF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 33-138) antibody
LEF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A8 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 10-37), (N-Term) antibody
LEF1 适用: 人 WB, IF 宿主: 兔 Polyclonal RB31989 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 51-100) antibody
LEF1 适用: 人, 犬, 豚鼠, Cow, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody
LEF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Xu, Zhao, Zhang, Xu, Yang, Wang, Liu: "Resveratrol Attenuates Hyperoxia-induced Oxidative Stress, Inflammation and Fibrosis and Suppresses Wnt/?-catenin Signaling in Lungs of Neonatal Rats." in: Clinical and experimental pharmacology & physiology, (2015) (PubMed).

Wang, Xie, Xie, Wan, Ma, Qi, Yang: "MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma." in: Neuroreport, Vol. 26, Issue 12, pp. 695-702, (2015) (PubMed).

Song, Man, Wang, Bai, Wei, Liu, Liu, Gu, Wang: "The Regenerating Spinal Cord of Gecko Maintains Unaltered Expression of ?-Catenin Following Tail Amputation." in: Journal of molecular neuroscience : MN, (2014) (PubMed).
抗原

LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

别名

LEF-1

背景

Synonyms: LEF-1, TCF1, TCF7L3, TCF1ALPHA, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1
Background: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP3. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

基因ID

51176

UniProt

Q9UJU2

途径

WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding