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PCNA 抗体 (Biotin)

This anti-PCNA antibody is a 小鼠 单克隆 antibody detecting PCNA in WB, IHC 和 IHC (fp). Suitable for 人, 小鼠, 大鼠 和 Saccharomyces cerevisiae.
产品编号 ABIN7659201
发货至: 中国

Quick Overview for PCNA 抗体 (Biotin) (ABIN7659201)

抗原

See all PCNA 抗体
PCNA (Proliferating Cell Nuclear Antigen (PCNA))

适用

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人, 小鼠, 大鼠, Saccharomyces cerevisiae

宿主

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小鼠

克隆类型

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单克隆

标记

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This PCNA antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

PC5
  • 原理

    PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)(PC5), Biotin conjugate

    产品特性

    Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Rat PCNA/Protein A fusion protein

    亚型

    IgG1, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Positive Control: Tonsil or reactive lymph node. MOLT-4, HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    PCNA (Proliferating Cell Nuclear Antigen (PCNA))

    别名

    PCNA

    背景

    Synonyms: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

    Gene Symbol: PCNA

    Tissue Expression: Proliferating cells

    分子量

    36 kDa

    基因ID

    5111

    UniProt

    P12004

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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