Recombinant Neurofilament 抗体 (Biotin)
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北京 101111
Quick Overview for Recombinant Neurofilament 抗体 (Biotin) (ABIN7657889)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Neurofilament (NF-H) (Neuronal Marker) (rNF421), Biotin conjugate
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产品特性
- This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human NEFH protein
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亚型
- IgG1
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anti-Neurofilament antibody
适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal SPM563 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal NF421 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Brain, Neuroblastoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Neurofilament
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背景
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Synonyms: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200
Gene Symbol: NEFH
Tissue Expression: Neuroendocrine cells|Neurons
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分子量
- 200 kDa
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基因ID
- 4744
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UniProt
- P12036
抗原
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