Neurofilament 抗体
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北京 101111
Quick Overview for Neurofilament 抗体 (ABIN3026907)
抗原
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Neurofilament Antibody (azide and preservative free)
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产品特性
- This mAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the Neurofilament antibody.
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亚型
- IgG1, kappa
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anti-Neurofilament antibody
适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal SPM563 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal NF421 unconjugated
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应用备注
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Optimal dilution of the Neurofilament antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Neurofilament
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背景
- This mAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
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UniProt
- P07196
抗原
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