Neurofilament 抗体
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北京 101111
Quick Overview for Neurofilament 抗体 (ABIN3023931)
抗原
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Anti-Neurofilament Antibody (azide and preservative free)
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human recombinant protein was used as the immunogen for this anti-Neurofilament antibody.
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亚型
- IgG1, kappa
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anti-Neurofilament antibody
适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal NF421 unconjugated
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应用备注
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The optimal dilution of the anti-Neurofilament antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Neurofilament
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背景
- This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
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UniProt
- P12036
抗原
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