HLA-B 抗体 (CF®740)
Quick Overview for HLA-B 抗体 (CF®740) (ABIN7656463)
抗原
See all HLA-B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- HLA-B(JOAN-1), CF740 conjugate
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产品特性
- HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Normal human peripheral blood lymphocytes
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亚型
- IgG3, kappa
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anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 241-362) antibody
HLA-B 适用: 人 ELISA, WB, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 2G7B10 unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 75-107), (N-Term) antibodyHLA-B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB47637 unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 25-308) antibodyHLA-B 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 1-362) antibodyHLA-B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 209-295) antibodyHLA-B 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4D4B6 unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 25-308) antibody (Biotin)HLA-B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal Biotin
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 197-306) antibodyHLA-B 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 243-308) antibodyHLA-B 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) antibodyHLA-B 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, B (HLA-B) (AA 132-143) antibodyVerified HLA-B 适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Human peripheral blood lymphocytes, tonsil or lymph node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))
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别名
- HLA-B
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背景
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Synonyms: HLA-B, HLAB, MHC Class I HLA heavy chain, Leukocyte antigen class I-B
Gene Symbol: HLA-B
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分子量
- 45 kDa
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基因ID
- 3106
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UniProt
- P03989
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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