HLA-A 抗体 (CF®740)
Quick Overview for HLA-A 抗体 (CF®740) (ABIN7656462)
抗原
See all HLA-A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- HLA-A (MHC I) (108-2C5), CF740 conjugate
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产品特性
- HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of thealpha1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of thealpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Normal human peripheral blood lymphocytes
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亚型
- IgG1, kappa
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anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 35-285) antibody
HLA-A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人, 小鼠, 大鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 1-365) antibodyHLA-A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 24-365) antibodyHLA-A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D6 unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 1-365) antibodyHLA-A 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 70-99) antibodyHLA-A 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人 FACS, IF 宿主: 小鼠 Monoclonal 108-2C5 unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibodyHLA-A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class I, A (HLA-A) (AA 82-106), (N-Term) antibodyHLA-A 适用: 人 WB, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: THP-1 and Jurkat cells. Tonsil or lymph node.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))
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别名
- HLA-A
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背景
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Synonyms: HLA-A, HLA class 1A, MHC class I HLA-A
Gene Symbol: HLA-A
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分子量
- ~41 kDa
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基因ID
- 3105
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UniProt
- P01889, P30443, P30499
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途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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