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Cytochrome C 抗体 (AA 1-80) (CF®640R)

Name: Cytochrome C 抗体 (AA 1-80) (CF®640R)
SKU: ABIN7652959
Availability: OutOfStock

This anti-Cytochrome C antibody is a 小鼠单克隆 antibody detecting Cytochrome C in WB, IHC, FACS 和 IHC (fp). Suitable for 人和大鼠.

产品编号 ABIN7652959

发货至: 中国

Quick Overview for Cytochrome C 抗体 (AA 1-80) (CF®640R) (ABIN7652959)

抗原

See all Cytochrome C (CYCS) 抗体

Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

适用

人, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This Cytochrome C antibody is conjugated to CF®640R

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

7H8-2C12 CYCS-1010

Discover our top product CYCS Primary Antibody

抗原表位
42

16

11

5

5

5

5

5

4

4

2

2

2

2

1

1

1

1

1

1

1
AA 1-80

原理

Cytochrome C (Mitochondrial Marker)(7H8.2C12 + CYCS/1010), CF640R conjugate

产品特性

Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

免疫原

Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c (7H8.2C12), Recombinant full-length human CYCS protein (CYCS/1010)

亚型

IgG2b kappa
anti-Cytochrome C, Somatic (CYCS) (AA 51-105) antibody
CYCS 适用: 人, 大鼠, 小鼠, 兔, 小鸡 WB, FACS, ELISA, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibody
CYCS 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibody
CYCS 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibody
CYCS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 13-99) antibody
CYCS 适用: 人, 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 5-100) antibody
CYCS 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal C2 unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 1-105) antibody
CYCS 适用: 人, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5E6B11 unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibody
CYCS 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 2-105) antibody
CYCS 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C, Somatic (CYCS) (AA 91-105), (C-Term) antibody
CYCS 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.25-0.5 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Western Blot 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

说明

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

限制

仅限研究用
状态

Liquid

浓度

0.1 mg/mL

缓冲液

PBS, 0.1 % BSA, 0.05 % azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Protect from light

储存条件

4 °C

储存方法

Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light

有效期

24 months
抗原

Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

别名

Cytochrome c

背景

Synonyms: CYC, CYCS, HCS, THC4
Gene Symbol: CYCS

分子量

15 kDa

基因ID

54205

UniProt

P99999

途径

Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity