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BCL2L1 抗体 (AA 3-14) (CF®740)

This anti-BCL2L1 antibody is a 小鼠 单克隆 antibody detecting BCL2L1 in WB, IHC 和 IHC (fp). Suitable for 人, 小鼠, 大鼠 和 Pig.
产品编号 ABIN7650013
发货至: 中国

Quick Overview for BCL2L1 抗体 (AA 3-14) (CF®740) (ABIN7650013)

抗原

See all BCL2L1 抗体
BCL2L1 (BCL2-Like 1 (BCL2L1))

适用

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人, 小鼠, 大鼠, Pig

宿主

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小鼠

克隆类型

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单克隆

标记

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This BCL2L1 antibody is conjugated to CF®740

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

2H12
  • 抗原表位

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    AA 3-14

    原理

    bcl-x(2H12), CF740 conjugate

    产品特性

    Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.

    亚型

    IgG2a
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    BCL2L1 (BCL2-Like 1 (BCL2L1))

    别名

    Bcl-x

    背景

    Synonyms: Bcl-x, Bcl-2-like protein 1, BCL2L1, Apoptosis regulator Bcl-X

    Gene Symbol: BCL2L1

    分子量

    27 kDa

    基因ID

    598

    UniProt

    Q07817

    途径

    Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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