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NF-kB p65 抗体 (pSer536)

Name: NF-kB p65 抗体 (pSer536)
SKU: ABIN730528
Availability: OutOfStock

Cited in 12+ publications. This 兔多克隆 anti-NF-kB p65 antibody specifically detects NF-kB p65 in WB, ELISA, IHC (p) 和 FACS. The antibody is reactive with 人, 小鼠, 大鼠, Cow 和小鸡 samples.

SeMet reversed activation of TLR4/NF-κB pathway induced by ESBL-E. (NF-kB p65 抗体 (pSer536))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (ABIN730528) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (NF-kB p65 抗体 (pSer536))

IHC

Mouse lung lysates probed with Rabbit Anti-NFkB p65 (Ser536) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (NF-kB p65 抗体 (pSer536))

Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C (NF-kB p65 抗体 (pSer536))

Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (NF-kB p65 抗体 (pSer536))

HL-60 cells probed with NFKB p65(Ser536) Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange) (NF-kB p65 抗体 (pSer536))

Necrostatin-1 attenuated neuroinflammatory changes in a rat neuropathic pain model. (NF-kB p65 抗体 (pSer536))

SeMet inhibited activation of TLR4/NF-κB pathway induced by ESBL-E. (NF-kB p65 抗体 (pSer536))

8 images

(12 references)

产品编号 ABIN730528

发货至: 中国

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Our Local Distributor

中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for NF-kB p65 抗体 (pSer536) (ABIN730528)

抗原

See all NF-kB p65 (NFkBP65) 抗体

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

适用

人, 小鼠, 大鼠, Cow, 小鸡

宿主

兔

克隆类型

多克隆

标记

This NF-kB p65 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Discover our top product NFkBP65 Primary Antibody

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pSer536

交叉反应

小鸡, Cow, 人, 小鼠, 大鼠

预测反应

Dog,Cow,Horse

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of(Ser536)

亚型

IgG
anti-Nuclear Factor-kB p65 (NFkBP65) (AA 51-100) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (Center) antibody
NFkBP65 适用: 人, 小鼠 WB, IF, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 19-306) antibody
NFkBP65 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 50-180) antibody
NFkBP65 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pThr435) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer281) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) antibody
KD Validated NFkBP65 适用: 人 WB, FACS, ICC, IF (p) 宿主: 兔 Monoclonal 23GB1490 unconjugated Recombinant Antibody

anti-Nuclear Factor-kB p65 (NFkBP65) (acLys314), (acLys315) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhuang, Liu, Barkema, Zhou, Xu, Ur Rahman, Liu, Kastelic, Gao, Han: "Selenomethionine Suppressed TLR4/NF-κB Pathway by Activating Selenoprotein S to Alleviate ESBL Escherichia coli-Induced Inflammation in Bovine Mammary Epithelial Cells and Macrophages." in: Frontiers in microbiology, Vol. 11, pp. 1461, (2020) (PubMed).

Liu, Li, Yang, Niu, Zhao, Zhao, Wang: "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." in: Biomedicine & pharmacotherapy, Vol. 96, pp. 1363-1370, (2018) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Singh, Koduru, Carlisle, Akhter, Liu, Schroder, Brandes, Ojcius: "NADPH oxidase 4 modulates hepatic responses to lipopolysaccharide mediated by Toll-like receptor-4." in: Scientific reports, Vol. 7, Issue 1, pp. 14346, (2017) (PubMed).

Yang, Liu, Jiang, Wang, Zhang: "Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model." in: Frontiers in pharmacology, Vol. 8, pp. 44, (2017) (PubMed).

Fu, Shi, Song, Xia, Su, Liu, Song, Song: "Tetrachlorobenzoquinone exhibits neurotoxicity by inducing inflammatory responses through ROS-mediated IKK/IκB/NF-κB signaling." in: Environmental toxicology and pharmacology, Vol. 41, pp. 241-50, (2016) (PubMed).

Zhao, Zheng, Hu, Shen, Li: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." in: Life sciences, Vol. 144, pp. 185-93, (2016) (PubMed).

Zhang, Zhao, Yuan, Wu, Jiang, Luo, Zhao: "Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway." in: International immunopharmacology, Vol. 38, pp. 167-74, (2016) (PubMed).

Gao, Guo, Wang, Lin, Yu, Sheng, Wu, Gong: "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." in: Acta histochemica, Vol. 118, Issue 6, pp. 606-14, (2016) (PubMed).

Yan, Wu, Li, Meng, Dai, Zheng, Fu: "Cyanidin-3-O-glucoside attenuates acute lung injury in sepsis rats." in: The Journal of surgical research, Vol. 199, Issue 2, pp. 592-600, (2015) (PubMed).

Zhao, Liu, Liu, Han, Zhao: "Betulin attenuates lung and liver injuries in sepsis." in: International immunopharmacology, Vol. 30, pp. 50-6, (2015) (PubMed).
抗原

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

别名

NFKB p65

背景

Synonyms: p65, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA
Background: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

基因ID

5970

UniProt

Q04206

途径

NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins