HNRNPD/AUF1 抗体 (AA 88-246)
Quick Overview for HNRNPD/AUF1 抗体 (AA 88-246) (ABIN7602590)
抗原
See all HNRNPD/AUF1 (HNRNPD) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 88-246
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原理
- Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband® (ABIN7602590). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human hnRNP D/AUF1/HNRNPD recombinant protein (Position: E88-N246).
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亚型
- IgG
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anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) antibody
HNRNPD 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (AA 1-306) antibodyHNRNPD 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (AA 1-306) antibodyHNRNPD 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (N-Term) antibodyHNRNPD 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (pSer83) antibodyHNRNPD 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (C-Term) antibodyHNRNPD 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 豚鼠, 斑马鱼, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (Ser83) antibodyHNRNPD 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (AA 1-306) antibodyHNRNPD 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (AA 18-355) antibodyHNRNPD 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD) (Internal Region) antibodyHNRNPD 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Arao Y, Kuriyama R, Kayama F, Kato S (August 2000). "A nuclear matrix-associated factor, SAF-B, interacts with specific isoforms of AUF1/hnRNP D". Arch. Biochem. Biophys. 380 (2): 228-36. 2. Sinsimer KS, Gratacós FM, Knapinska AM, Lu J, Krause CD, Wierzbowski AV, Maher LR, Scrudato S, Rivera YM, Gupta S, Turrin DK, De La Cruz MP, Pestka S, Brewer G (September 2008). "Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decay". Mol. Cell. Biol. 28 (17): 5223-37. 3. Panda AC, Abdelmohsen K, Yoon JH, Martindale JL, Yang X, Curtis J, Mercken EM, Chenette DM, Zhang Y, Schneider RJ, Becker KG, de Cabo R, Gorospe M (Aug 2014). "RNA-binding protein AUF1 promotes myogenesis by regulating MEF2C expression levels". Mol Cell Biol. 34 (16): 3106-19. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))
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别名
- HNRNPD
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背景
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Synonyms: Heterogeneous nuclear ribonucleoprotein D0, hnRNP D0, AU-rich element RNA-binding protein 1, HNRNPD, AUF1, HNRPD
Background: Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) also known as AU-rich element RNA-binding protein 1 (AUF1) is a protein that in humans is encoded by the HNRNPD gene. It is mapped to 4q21.22. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
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分子量
- 37-45 kDa
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基因ID
- 3184
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UniProt
- Q14103
抗原
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