BARD1 抗体 (AA 709-758)
Quick Overview for BARD1 抗体 (AA 709-758) (ABIN7602360)
抗原
See all BARD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 709-758
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原理
- Anti-BARD1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-BARD1 Antibody Picoband® (ABIN7602360). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human BARD1 recombinant protein (Position: P709-A758).
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亚型
- IgG
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anti-BRCA1 Associated RING Domain 1 (BARD1) (AA 101-200) antibody
BARD1 适用: 人 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BRCA1 Associated RING Domain 1 (BARD1) (AA 658-757) antibodyBARD1 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 2A11 unconjugated
anti-BRCA1 Associated RING Domain 1 (BARD1) (N-Term) antibodyBARD1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-BRCA1 Associated RING Domain 1 (BARD1) (N-Term) antibodyBARD1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-BRCA1 Associated RING Domain 1 (BARD1) (AA 428-777) antibodyBARD1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-BRCA1 Associated RING Domain 1 (BARD1) antibodyBARD1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-BRCA1 Associated RING Domain 1 (BARD1) antibodyBARD1 适用: 人 WB, IP, DB 宿主: 小鼠 Monoclonal 2059C4a unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Daza-Martin, M., Starowicz, K., Jamshad, M., Tye, S., Ronson, G. E., MacKay, H. L., Chauhan, A. S., Walker, A.K., Stone, H. R., Beesley, J. F. J., Coles, J. L., Garvin, A. J., Stewart, G. S., McCorvie, T. J., Zhang, X., Densham, R. M., Morris, J. R. Isomerization of BRCA1-BARD1 promotes replication fork protection. Nature 571: 521-527, 2019. 2. Dong, Y., Hakimi, M.-A., Chen, X., Kumaraswamy, E., Cooch, N. S., Godwin, A. K., Shiekhattar, R. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair. Molec. Cell 12: 1087-1099, 2003. 3. Irminger-Finger, I., Leung, W.-C., Li, J., Dubois-Dauphin, M., Harb, J., Feki, A., Jefford, C. E., Soriano, J. V., Jaconi, M., Montesano, R., Krause, K.-H. Identification of BARD1 as mediator between proapoptotic stress and p53-dependent apoptosis. Molec. Cell 8: 1255-1266, 2001. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- BARD1 (BRCA1 Associated RING Domain 1 (BARD1))
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别名
- BARD1
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背景
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Synonyms: RNA polymerase II-associated factor 1 homolog, hPAF1, Pancreatic differentiation protein 2, PAF1, PD2
Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.
Background: BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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分子量
- 87 kDa
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基因ID
- 580
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UniProt
- Q99728
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途径
- DNA Damage Repair
抗原
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