KAP1 抗体 (AA 699-835)
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北京 101111
Quick Overview for KAP1 抗体 (AA 699-835) (ABIN7602311)
抗原
See all KAP1 (TRIM28) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 699-835
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原理
- Anti-KAP1/TRIM28 Antibody (monoclonal, 9E3)
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特异性
- No cross reactivity with other proteins.
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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预测反应
- Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse,rat KAP1.
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产品特性
- Anti-KAP1/TRIM28 Antibody.(monoclonal, 9E3)-2. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.
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亚型
- IgG2a
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
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别名
- TRIM28
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背景
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Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
Gene Full Name: tripartite motif containing 28
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分子量
- 100 kDa
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基因ID
- 10155
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UniProt
- Q13263
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途径
- Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
抗原
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