Flotillin 2 抗体 (AA 169-344)
Quick Overview for Flotillin 2 抗体 (AA 169-344) (ABIN7600242)
抗原
See all Flotillin 2 (FLOT2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 169-344
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原理
- Anti-Flotillin 2 Antibody Picoband® (monoclonal, 4D8A3)
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Flotillin 2 Antibody Picoband® (monoclonal, 4D8A3) (ABIN7600242). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both and rat Flotillin 2.
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亚型
- IgG2b
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anti-Flotillin 2 (FLOT2) (C-Term) antibody
FLOT2 适用: 人 WB, IHC (p), IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 1-379) antibodyFLOT2 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3G6 unconjugated
anti-Flotillin 2 (FLOT2) (AA 1-379) antibodyFLOT2 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 169-344) antibodyFLOT2 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 239-428) antibodyFLOT2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (C-Term) antibodyVerified FLOT2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 1-379) antibodyFLOT2 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) antibodyFLOT2 适用: 化学剂 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) antibodyFLOT2 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., Lodish, H. F. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J. Biol. Chem. 272: 13793-13802, 1997. 2. Li, Y., Martin, B. R., Cravatt, B. F., Hofmann, S. L. DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells. J. Biol. Chem. 282: 523-530, 2012. 3. Schroeder, W. T., Siciliano, M. J., Stewart-Galetka, S. L., Duvic, M. The human gene for an epidermal surface antigen (M17S1) is located at 17q11-12. Genomics 11: 481-482, 1991. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Flotillin 2 (FLOT2)
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别名
- FLOT2
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背景
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Synonyms: T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5
Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Background: FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.
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分子量
- 49 kDa
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基因ID
- 2319
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UniProt
- Q14254
抗原
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