INSIG1 抗体 (AA 1-87)
Quick Overview for INSIG1 抗体 (AA 1-87) (ABIN7599566)
抗原
See all INSIG1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-87
-
原理
- Anti-INSIG1 Antibody Picoband®
-
交叉反应 （详细）
- No cross reactivity with other proteins.
-
产品特性
- Anti-INSIG1 Antibody Picoband® (ABIN7599566). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human INSIG1 recombinant protein (Position: M1-R87).
-
亚型
- IgG
-
-
anti-Insulin Induced Gene 1 (INSIG1) (AA 1-87) antibody
INSIG1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (Internal Region) antibodyINSIG1 适用: 人, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (AA 63-112) antibodyINSIG1 适用: 人, 小鼠, 大鼠, Cow, 山羊, 马, 兔, 犬, 豚鼠, Pig, 猴, Hamster, 非洲爪蟾, Bat, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (AA 36-63), (N-Term) antibodyINSIG1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21689 unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (Middle Region) antibodyINSIG1 适用: 人, 小鼠, 大鼠, Cow, 山羊, 马, 兔, 犬, 豚鼠, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (AA 71-120) antibodyINSIG1 适用: 人, 小鼠, 大鼠, Cow, 山羊, 马, 兔, 绵羊, Pig, Hamster, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (C-Term) antibodyINSIG1 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (C-Term) antibodyINSIG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (Middle Region) antibodyINSIG1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin Induced Gene 1 (INSIG1) (Middle Region) antibodyINSIG1 适用: 人, 小鼠, 大鼠, Cow, 山羊, 马, 兔, 犬, 豚鼠, 绵羊, Pig, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Diamond, R. H., Du, K., Lee, V. M., Mohn, K. L., Haber, B. A., Tewari, D. S., Taub, R. Novel delayed-early and highly insulin-induced growth response genes: identification of HRS, a potential regulator of alternative pre-mRNA splicing. J. Biol. Chem. 268: 15185-15192, 1993. 2. Engelking, L. J., Liang, G., Hammer, R. E., Takaishi, K., Kuriyama, H., Evers, B. M., Li, W.-P., Horton, J. D., Goldstein, J. L., Brown, M. S. Schoenheimer effect explained--feedback regulation of cholesterol synthesis in mice mediated by Insig proteins. J. Clin. Invest. 115: 2489-2498, 2005. 3. Gong, Y., Lee, J. N., Brown, M. S., Goldstein, J. L., Ye, J. Juxtamembranous aspartic acid in Insig-1 and Insig-2 is required for cholesterol homeostasis. Proc. Nat. Acad. Sci. 103: 6154-6159, 2006. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- INSIG1 (Insulin Induced Gene 1 (INSIG1))
-
别名
- INSIG1
-
背景
-
Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Tissue Specificity: Expressed in all tissues.
Background: Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.
-
分子量
- 66 kDa
-
基因ID
- 3638
-
UniProt
- O15503
-
途径
- ER-Nucleus Signaling
抗原
-
