GDNF 抗体 (AA 54-66)
Quick Overview for GDNF 抗体 (AA 54-66) (ABIN7581880)
抗原
See all GDNF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 54-66
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原理
- A Rabbit Polyclonal Antibody to proGDNF
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序列
- (C)PEDYPDQFDD VMD
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特异性
- Pro-domain of the GDNF protein
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交叉反应 （详细）
- The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF.
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预测反应
- Rat,mouse - identical
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产品特性
- Anti-proGDNF Antibody (ABIN7581880) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize proGDNF from human, mouse, and rat samples. The antibody is specific for the pro-domain region of GDNF and will not react with mature GDNF.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)PEDYPDQFDDVMD, corresponding to amino acid residues 54-66 of human GDNF (precursor)
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亚型
- IgG
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anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 121-211) antibody
GDNF 适用: 人, 小鼠, 大鼠, Cow, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) antibodyGDNF 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 兔 Monoclonal 11D2 unconjugated Recombinant Antibody
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 142-158), (Middle Region) antibodyGDNF 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 8-36) antibodyGDNF 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 109-211) antibodyGDNF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 192-207), (Mature) antibodyGDNF 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 121-211) antibody (FITC)GDNF 适用: 人, 小鼠, 大鼠, Cow, 兔 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) antibodyGDNF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) antibodyGDNF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 79-217) antibodyGDNF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
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别名
- GDNF
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背景
- Glial cell line-derived neurotrophic factor precursor,Glial-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily. GDNF signals through a multi-component receptor system, composed of a RET protooncogene and one of the four α1-α4 receptors1.GDNF promotes survival of various neuronal cells, including motoneurons2,3, Purkinje cells and sympathetic neurons4. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake5. Cells that express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells6, neurons, pinealocytes, and skeletal muscle cells7.In vivo, following transection of facial motor neuron axons, locally applied GDNF has been shown to rescue virtually all damaged neurons from death8. GDNF may be of clinical relevance in the treatment of Parkinson's disease that is characterized by progressive degeneration of midbrain dopaminergic neurons9,10.Recently, it has been hypothesized that functional, carboxy-terminally amidated peptides are processed from the GDNF precursor upon proteolytic cleavage by furin-like endopeptidase11,12,13. Those different peptides (a 5-mer and 11-mer) have not been isolated endogenously to date. However, the rat 11-mer sequence (named brain excitatory peptide, BEP) significantly induced synaptic excitability and possessed some dopaminergic activities in vitro (thus named dopamine neuron stimulating peptides, DNSP)13. Furthermore, the human 11-mer sequence (named DNSP-11) exhibits neurotrophic-like properties13.Thus, the role of the full proDomain of GDNF, which is a product of proteolytic cleavage of proGDNF, is not clearly understood yet.
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基因ID
- 2668
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UniProt
- P39905
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途径
- RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
抗原
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