Recombinant EPH Receptor A3 抗体
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北京 101111
Quick Overview for Recombinant EPH Receptor A3 抗体 (ABIN7543438)
抗原
See all EPH Receptor A3 (EPHA3) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-EpHA3 [IIIA4], Mouse IgG1, kappa
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特异性
- The antibody binds to the native EphA3 globular ephrin-binding domain. The antibody targets a site closely adjacent to the heterotetramerization site on the N-terminal of EPHA3's extracellular domain adjacent to the ligand-binding site and has a high affinity for EPHA3.
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交叉反应
- 小鼠
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was prepared by intraperitoneal immunization of a BALB/c mouse with LK63 cells.
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亚型
- IgG1 kappa
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anti-EPH Receptor A3 (EPHA3) (AA 202-326) antibody
EPHA3 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 896-928) antibodyEPHA3 适用: 人, Hamster WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 202-326) antibodyEPHA3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 896-928), (C-Term) antibodyEPHA3 适用: 人, Hamster WB, IHC (p) 宿主: 兔 Polyclonal RB1585 unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 488-500), (Extracellular) antibodyEPHA3 适用: 人 WB, IHC, FACS, IF, LCI 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (C-Term) antibodyEPHA3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 38-375) antibodyEPHA3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 751-983) antibodyEPHA3 适用: 人 ELISA 宿主: 小鼠 Monoclonal 6C1B6 unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 115-144) antibodyEPHA3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A3 (EPHA3) (AA 401-500) antibodyEPHA3 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody was used for detection of hek expressed on LK63 cells by flow cytometry. Hek antigen was immunoprecipitated using the antibody from 125I-labeled LK63 cell lysates (Boyd et al., 1992, PMID: 1737782). The specificity of the chimeric version of the antibody was confirmed by ELISA analysis (US8664365). In a LK63 xenograft model, administration of the antibody inhibited the tumor growth, decreasing the spreading from the bone marrow to the spleen and other organs and increases the latency of the disease. Similar effects were observed in LK63 EPHA3 knock down xenografts. Further, antibody treatment of a xenograft model using EPHA3-transfected Reh, showed reduction in the bone marrow engraftment and increases the latency of the disease (Charmsaz et al., 2016, PMID: 27922598). The antibody bound to the native EphA3 globular ephrin-binding domain with subnanomolar affinity (KD ~ 0.5 nM) (Smith et al., 2004, PMID: 14660665). The antibody specifically stained EphA3 in 95 % of human tumor tissues from a broad range of cancer types (Vail et al. 2014, PMID: 25125683). The humanized version of the antibody (KB004), was tested in a multi-center Phase 1/2 trial, in patients with EphA3-positive hematologic neoplasms, including Chronic Myelogenous Leukemia, Acute Myeloid Leukemia, Acute lymphoblastic leukemia, Myelodysplastic Syndrome.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- EPH Receptor A3 (EPHA3)
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别名
- EpHA3
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背景
- Ephrin type-A receptor 3, EPH-like kinase 4, EK4, hEK4, HEK, Human embryo kinase, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, Eph-like tyrosine kinase 1, III.A4
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UniProt
- P29320
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途径
- RTK signaling, Regulation of Cell Size
抗原
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