ATM 抗体 (AA 1974-1988)
(7 references)
Quick Overview for ATM 抗体 (AA 1974-1988) (ABIN7540580)
抗原
See all ATM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1974-1988
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Supplier Product No.
- 600-401-398
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Supplier
- Rockland
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原理
- ATM Protein Kinase S1981 Antibody
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交叉反应 （详细）
- This polyclonal anti-ATM antibody detects native and over-expressed proteins found in various tissues and extracts.
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纯化方法
- This affinity-purified antibody is directed against human ATM and is useful in determining its presence in various assays.
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过滤
- Sterile filtered
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免疫原
- This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide S-L-A-F-E-E-G-S-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM conjugated to KLH using maleimide.
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亚型
- IgG
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anti-Ataxia Telangiectasia Mutated (ATM) (AA 1974-1988), (pSer1981) antibody
ATM 适用: 人 WB, IHC, ELISA, IF, IP, FACS, FM 宿主: 小鼠 Monoclonal 10H11-E12 unconjugated
anti-Ataxia Telangiectasia Mutated (ATM) (AA 1974-1988), (pSer1981) antibodyATM 适用: 人 WB, IHC, ELISA, IF, IP, FACS, FM 宿主: 小鼠 Monoclonal 10H11-E12 unconjugated
anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibodyATM 适用: 人, 小鼠 WB, IHC, ELISA, IF, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ataxia Telangiectasia Mutated (ATM) (AA 2681-2750) antibodyATM 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ataxia Telangiectasia Mutated (ATM) (AA 2484-2748) antibodyATM 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ataxia Telangiectasia Mutated (ATM) antibodyATM 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Monoclonal 4E11 unconjugated Recombinant Antibody
anti-Ataxia Telangiectasia Mutated (ATM) (AA 1974-1988), (pSer1981) antibodyATM 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7C10D8 unconjugated
anti-Ataxia Telangiectasia Mutated (ATM) (AA 1974-1988), (pSer1981) antibody (HRP)ATM 适用: 人 WB, IHC, ELISA, FM 宿主: 小鼠 Monoclonal 10H11-E12 HRP
anti-Ataxia Telangiectasia Mutated (ATM) (AA 486-742) antibodyATM 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ataxia Telangiectasia Mutated (ATM) antibodyATM 适用: 人 IHC, ELISA, FACS 宿主: 兔 Monoclonal 3G11 unconjugated Recombinant Antibody
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: User Optimized
Western_Blot_Dilution: 1:500- 1:2,000
Other: User Optimized
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说明
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Suggested Applications: FC, IF, Multiplex
Affinity purified rabbit anti-ATM has been tested by ELISA, immunohistochemistry, western blotting and suitable for biological assays against both the native and recombinant forms of the protein. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Hypoxia-Targeted Drug Q6 Induces G2-M Arrest and Apoptosis via Poisoning Topoisomerase II under Hypoxia." in: PloS one, Vol. 10, Issue 12, pp. e0144506, (2019) (PubMed).
: "Pathological neoangiogenesis depends on oxidative stress regulation by ATM." in: Nature medicine, Vol. 18, Issue 8, pp. 1208-16, (2013) (PubMed).
: "Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2374-82, (2012) (PubMed).
: "Essential roles of Xenopus TRF2 in telomere end protection and replication." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 16, Issue 6, pp. 728-39, (2011) (PubMed).
: "HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair." in: The Journal of cell biology, Vol. 193, Issue 1, pp. 81-95, (2011) (PubMed).
: "Deep senescent human fibroblasts show diminished DNA damage foci but retain checkpoint capacity to oxidative stress." in: FEBS letters, Vol. 580, Issue 28-29, pp. 6669-73, (2006) (PubMed).
: "ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1." in: Molecular and cellular biology, Vol. 25, Issue 13, pp. 5363-79, (2005) (PubMed).
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: "Hypoxia-Targeted Drug Q6 Induces G2-M Arrest and Apoptosis via Poisoning Topoisomerase II under Hypoxia." in: PloS one, Vol. 10, Issue 12, pp. e0144506, (2019) (PubMed).
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- ATM (Ataxia Telangiectasia Mutated (ATM))
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别名
- ATM
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背景
- Rabbit anti-ATM antibody, rabbit anti-ATM protein kinase S1891 antibody, rabbit anti-ATM protein kinase antibody, Ataxia telangiectasia mutated, A-T mutated, Serine-protein kinase ATM,ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. See Bakkenist, C. J. & Kastan, M. B. Nature 421, 499-506 (2003) for a complete presentation of this antibody's specificity and utility.
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基因ID
- 472
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NCBI登录号
- NP_000042
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UniProt
- Q13315
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途径
- p53 Pathway, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
抗原
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