Recombinant VEGFA 抗体
Our Local Distributor
北京 101111
Quick Overview for Recombinant VEGFA 抗体 (ABIN7540553)
抗原
See all VEGFA 抗体抗体类型
片段
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 400-001-mt4
-
Supplier
- Rockland
-
原理
- Recombinant Anti-VEGF Fab Antibody
-
纯化方法
- Humanized Recombinant VEGF-A Fab fragment was expressed in CHO cells.
-
纯度
- > 95 % by SDS-PAGE analysis
-
过滤
- Sterile filtered
-
亚型
- IgG1
-
-
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibody
VEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibodyVEGFA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 6G5B5 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F7 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 6G5A10 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 人 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
-
ELISA_Dilution: User Optimized
Flow_Cytometry_Dilution: User Optimized
Western_Blot_Dilution: User Optimized
-
说明
-
Humanized Recombinant Anti-VEGFA Fab fragment Antibody has been tested for use in Flow Cytometry, Western Blot, and ELISA. This antibody recognizes structured VEGF-A and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: None -
储存液
- Without preservative
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C. Dilute only prior to immediate use.
-
有效期
- 6 months
-
-
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
-
别名
- VEGF-A
-
背景
- Anti-VEGF-A Antibody, Ranibizumab, Lucentis, Fab fragment of Bevacizumab or Avastin, Vascular endothelial growth factor A, VEGF A, VEGFA, Vascular permeability factor, VPF,VEGF is a potent mitogen in embryonic and somatic angiogenesis with specificity for vascular endothelial cells. VEGF forms homodimers and exists in four different isoforms. Overall, the VEGF monomer resembles that of PDGF, but its N-terminal segment is helical rather than extended. VEGF shares homologies of about 21 % and 24 % respectively with the A and B chains of human platelet-derived growth factor (PDGF), and has complete conservation of the eight cysteine residues found in both mature PDGF chains. The cysteine knot motif is a common feature of this domain. The homology is not reflected in function, however, since the cell types responsive to VEGF are distinct from those responsive to homo- and heterodimers of the PDGF chains. This protein is a glycosylated mitogen that acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. VEGF-A also has been shown to have effects on a number of other cell types (e.g. stimulation of monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ). VEGF-A is also a vasodilator, it increases microvascular permeability, and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Antibody against VEGF-A (Bevacizumab or brand name Avastin) is approved for treatment of cancer by inhibiting angiogenesis. Ranibizumab, a Fab fragment of Avastin or so-called Lucentis is approved to treat the "wet" type of age-related macular degeneration (AMD, also ARMD), diabetic retinopathy, and macular edema due to branch retinal vein occlusion or central retinal vein occlusion. This antibody is made from a humanized Fab fragment making it specific, efficient, and effective. Humanized Recombinant Anti-VEGF-A Fab fragment Antibody is useful for researchers interested in angiogenesis associated diseases such as cancer or wet AMD.
-
基因ID
- 7422
-
NCBI登录号
- NP_001020537
-
UniProt
- P15692
-
途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
-
