电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

VEGFA 抗体

VEGFA 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465403
发货至: 中国
  • 抗原 See all VEGFA 抗体
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    适用
    • 155
    • 126
    • 98
    • 24
    • 20
    • 19
    • 19
    • 16
    • 12
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 203
    • 54
    • 13
    • 1
    克隆类型
    • 214
    • 56
    • 1
    多克隆
    标记
    • 122
    • 41
    • 25
    • 12
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This VEGFA antibody is un-conjugated
    应用范围
    • 206
    • 92
    • 65
    • 65
    • 52
    • 32
    • 27
    • 22
    • 22
    • 16
    • 15
    • 12
    • 10
    • 8
    • 8
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Human VEGF
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human VEGF165.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product VEGFA Primary Antibody
  • 应用备注
    Approved: ELISA, Neut (0.05 - 0.1 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5-5.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hVEGF (10 ng/mL), a concentration of 0.05-0.1 μg/mL of this antibody is required. ELISA: To detect hVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VEGF (LS-C104615) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    别名
    VEGFA / VEGF (VEGFA 产品)
    别名
    MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, vegf antibody, vegfa antibody, wu:fj82c06 antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vpf antibody, vefg antibody, si:dkey-14d8.3 antibody, wu:fd42e02 antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor Aa antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor Ab antibody, VEGFA antibody, Vegfa antibody, vegfaa antibody, vegfa.L antibody, vegfa antibody, vegfab antibody
    背景
    Name/Gene ID: VEGFA
    Family: PDGF

    Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1
    基因ID
    7422
    UniProt
    P15692
    途径
    RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
客服