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- 抗原 See all IgE 抗体
- IgE
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IgE antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS)
- 原理
- Anti-Hu IgE APC
- 特异性
- The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
- 纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- Purified human IgE.
- 克隆位点
- 4H10
- 亚型
- IgG1
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anti-IgE antibody (PE)
适用: 人 FACS 宿主: 小鼠 Monoclonal 4H10 PE
anti-IgE antibody (FITC)适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal BE5 FITC
anti-IgE antibody (PE)适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal BE5 PE
anti-IgE antibody (Biotin)适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal BE5 Biotin
anti-IgE (AA 1-421) antibody适用: 小鼠 WB, ICC, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-IgE antibody (Biotin)适用: 人 ELISA, ICC, IHC, FACS 宿主: 兔 Monoclonal RM122 Biotin
anti-IgE antibody适用: 人 ELISA, ICC, IHC, FACS 宿主: 兔 Monoclonal RM122 unconjugated
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- 应用备注
- Flow cytometry: Recommended dilution: 0.5-3 μg/mL
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- 抗原
- IgE
- Abstract
- IgE 产品
- 别名
- IGH2 antibody, IgE antibody, Gm900 antibody, immunoglobulin heavy chain (epsilon polypeptide) antibody, immunoglobulin heavy constant epsilon antibody, Immunoglobulin heavy constant epsilon antibody, Ighe antibody, IGHE antibody
- 背景
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
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