Recombinant SSEA-4 抗体
SSEA-4
适用: 人
ELISA, IHC (fro), WB
宿主: 兔
Monoclonal
E2-F8
unconjugated
Recombinant Antibody
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- 抗原 See all SSEA-4 products
- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This SSEA-4 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
- 原理
- Anti-SSEA-4 Antibody (clone E2-F8)
- 特异性
- Positive Control: Colon cancer tissue
- 产品特性
- SSEA-4 F8 antibody is an unconjugated recombinant rabbit monoclonal antibody to Stage-Specific Embryonic Antigen-4 (SSEA-4). Validated for ELISA, IHC-FR and immunoblotting applications.
- 纯化方法
- Purified by Protein A or Protein G affinity chromatography
- 纯度
- >95 %
- 内毒素水平
- <0.050 EU/mg as determined by Limulus Amebocyte Lysate (LAL) chromogenic endotoxin assay
- 克隆位点
- E2-F8
- 亚型
- IgG1
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS buffer ( pH 7.5)
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store frozen in aliquots at -20°C for up to one year, or at 4°C for up to one week
- 有效期
- 12 months
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- 抗原
- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
- 别名
- SSEA4 (SSEA-4 产品)
- 背景
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Stage-specific embryonic antigen-4 (SSEA-4) is a ganglioside which consists of a glycosphingolipid (GSL) containing a terminal sialic acid residue (N-acetylneuraminic acid). SSEA-4 expression changes both qualitatively and quantitatively during development, differentiation and in tumorigenesis. During human development, SSEA-4 is first observed on pluripotent cells of the inner cell mass and is subsequently lost upon differentiation (Tondeur et al. 2008). In addition, human germ stem cells in the testis and ovary express SSEA-4 (Harichandan et al. 2013; Virant-Klun et al. 2013).
SSEA-4 has been found to be over-expressed in a number of cancers and has been correlated with disease progression. Studies by the Buhring group (Sivasubramaniyan et al, 2015) proposed SSEA-4 as a novel marker to identify heterogeneous, invasive subpopulations of tumor cells. Importantly, they also demonstrated that increased cell-surface SSEA-4 expression is associated with the loss of cell–cell interactions and the gain of a migratory phenotype, suggesting an important role of SSEA-4 in cancer invasion by influencing cellular adhesion to the extracellular matrix.
Studies of SSEA-4 expression and function have often been inhibited by the lack of high quality reagents. Due to the glycan nature of the antigen almost all antibodies that have been developed are of IgM or IgG3 classes, which tend to be of low affinity and can be difficult to handle.
Glykogen have overcome these reagent challenges by using their proprietary immunisation methods to develop Clone F8, a recombinant monoclonal rabbit IgG antibody. The availability of this antibody will facilitate studies into SSEA-4 expression and function, both in stem cell biology and in cancer studies. - 分子量
- 143 kD
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