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Recombinant Nucleolin 抗体

NCL 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-623R unconjugated Recombinant Antibody
产品编号 ABIN7477541
发货至: 中国
  • 抗原 See all Nucleolin (NCL) 抗体
    Nucleolin (NCL)
    抗体类型
    Recombinant Antibody
    适用
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    宿主
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    克隆类型
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    单克隆
    标记
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    This Nucleolin antibody is un-conjugated
    应用范围
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    Immunohistochemistry (IHC)
    免疫原
    Recombinant full-length human NCL protein
    克隆位点
    MSVA-623R
    亚型
    IgG
    Top Product
    Discover our top product NCL Primary Antibody
  • 应用备注
    IHC 1:100-1:200
    说明

    Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.

    Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.

    实验流程
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    限制
    仅限研究用
  • 状态
    Liquid
    储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原
    Nucleolin (NCL)
    别名
    Nucleolin (NCL 产品)
    别名
    C23 antibody, B530004O11Rik antibody, D0Nds28 antibody, D1Nds28 antibody, Nucl antibody, wu:fa12d03 antibody, zgc:152810 antibody, nucl antibody, LOC100282923 antibody, LOC100283058 antibody, nucleolin antibody, nucleolin S homeolog antibody, NCL antibody, Ncl antibody, ncl.S antibody, ncl antibody, LOC100195537 antibody, LOC100282923 antibody, LOC100283058 antibody
    背景
    NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P19338
    途径
    Ribonucleoprotein Complex Subunit Organization
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