H2AFX 抗体 (C-Term)
Quick Overview for H2AFX 抗体 (C-Term) (ABIN7464767)
抗原
See all H2AFX 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
交叉反应
- 人, 小鼠, 大鼠
-
纯化方法
- Purified by antigen-affinity chromatography.
-
免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.
-
亚型
- IgG
-
-
-
-
应用备注
- WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
-
说明
-
Positive Control: mouse brain , rat brain , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , HCT116 (30uM cisplatin for 24hr)
Validation: Orthogonal
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.81 mg/mL
-
缓冲液
- 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
-
- H2AFX (H2A Histone Family, Member X (H2AFX))
-
别名
- H2A histone family member X
-
背景
- H2A histone family member X , H2A.X , H2A/X , H2AX,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
-
分子量
- 15 kDa
-
基因ID
- 3014
-
UniProt
- P16104
-
途径
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原
-