PCNA 抗体 (C-Term)
Quick Overview for PCNA 抗体 (C-Term) (ABIN7455223)
抗原
See all PCNA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Rabbit anti-PCNA Antibody, Affinity Purified
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预测反应
- Rat,Bovine
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纯化方法
- Affinity Purified
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免疫原
- between AA 225 and C-term
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亚型
- IgG
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应用备注
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IHC-IF: 1:1,000 - 1:5,000
IHC: 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 10 μg/mg lysate
WB: 1:5,000 - 1:20,000
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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别名
- PCNA
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背景
- Background: PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
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基因ID
- 5111
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NCBI登录号
- NP_872590
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UniProt
- P12004
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
抗原
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