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PCNA 抗体 (AA 75-125)

This anti-PCNA antibody is a 兔 多克隆 antibody detecting PCNA in WB, IP 和 IHC (fp). Suitable for 人 和 小鼠.
产品编号 ABIN7454604
发货至: 中国

Quick Overview for PCNA 抗体 (AA 75-125) (ABIN7454604)

抗原

See all PCNA 抗体
PCNA (Proliferating Cell Nuclear Antigen (PCNA))

适用

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人, 小鼠

宿主

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克隆类型

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  • 1
多克隆

标记

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This PCNA antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
  • 抗原表位

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    AA 75-125

    原理

    Rabbit anti-PCNA Antibody, Affinity Purified

    预测反应

    Rat,Chicken,Bovine

    纯化方法

    Affinity Purified

    免疫原

    between AA 75 and 125

    亚型

    IgG
  • 应用备注

    IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    IP: 2 - 5 μg/mg lysate

    WB: 1:1,000 - 1:10,000

    限制

    仅限研究用
  • 浓度

    1000 μg/mL

    缓冲液

    Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    有效期

    12 months
  • 抗原

    PCNA (Proliferating Cell Nuclear Antigen (PCNA))

    别名

    PCNA

    背景

    Background: PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

    基因ID

    5111

    NCBI登录号

    NP_872590

    UniProt

    P12004

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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