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Bcl-2 抗体 (AA 50-100)

The 兔 多克隆 anti-Bcl-2 antibody (ABIN7454495) specifically detects Bcl-2 in IP 和 IHC (fp). The antibody is reactive with 人 samples.
产品编号 ABIN7454495
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Bcl-2 抗体 (AA 50-100) (ABIN7454495)

抗原

See all Bcl-2 (BCL2) 抗体
Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

适用

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宿主

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克隆类型

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多克隆

标记

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This Bcl-2 antibody is un-conjugated

应用范围

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Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
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    AA 50-100

    原理

    Rabbit anti-Bcl-2 Antibody, Affinity Purified

    纯化方法

    Affinity Purified

    免疫原

    Between AA 50 and 100

    亚型

    IgG
  • 应用备注

    IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    IP: 2 - 10 μg/mg lysate

    WB: Not recommended. A303-675A has not performed satisfactorily when used for WB of Bcl-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Bcl-2.

    限制

    仅限研究用
  • 浓度

    1000 μg/mL

    缓冲液

    Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    有效期

    12 months
  • 抗原

    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

    别名

    Bcl-2

    背景

    Background: B-cell CLL/lymphoma 2 (Bcl-2) is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl-2, such as in the case of translocation of Bcl-2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma [taken from NCBI Entrez Gene (Gene ID: 596)].

    基因ID

    596

    NCBI登录号

    NP_000624

    UniProt

    P10415

    途径

    MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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