MYBL2 抗体 (AA 450-500)
Quick Overview for MYBL2 抗体 (AA 450-500) (ABIN7454417)
抗原
See all MYBL2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 450-500
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原理
- Rabbit anti-B-Myb Antibody, Affinity Purified
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纯化方法
- Affinity Purified
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免疫原
- between AA 450 and 500
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亚型
- IgG
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应用备注
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IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 5 μg/mg lysate
WB: Not recommended. A301-655A has not performed satisfactorily when used for WB of B-Myb in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of B-Myb.
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))
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别名
- B-Myb
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背景
- Background: B-myb is transcription factor involved in multiple cellular processes such as cell survival, proliferation, and differentiation. B-myb was identified in a low-stringency screen of a T-cell cDNA library probed with a C-myb probe. C-myb is a cellular proto-oncogene highly conserved in vertebrates and invertebrates. The chicken c-myb proto-oncogene was the first ortholog discovered. Investigation of the origins of the avian myeloblastosis virus (AMV) led to the discovery that the AMV genome contained transduced sequences from normal chicken cellular DNA that resulted in a viral oncogenic version (v-myb) of the c-myb gene. B-myb is cell cycle regulated and expressed in all proliferating cells. Recent studies show that it is the only myb family member detected in embryonic stem cells and is essential to cell cycle progression and genomic stability.
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基因ID
- 4605
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NCBI登录号
- NP_002457
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UniProt
- P10244
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, Chromatin Binding
抗原
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