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RPA1 抗体 (AA 350-400)

This anti-RPA1 antibody is a 兔 多克隆 antibody detecting RPA1 in WB, IP 和 IHC (fp). Suitable for 人 和 小鼠.
产品编号 ABIN7453556
发货至: 中国

Quick Overview for RPA1 抗体 (AA 350-400) (ABIN7453556)

抗原

See all RPA1 抗体
RPA1 (Replication Protein A1, 70kDa (RPA1))

适用

  • 80
  • 23
  • 17
  • 7
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
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  • 1
人, 小鼠

宿主

  • 65
  • 16
  • 1
  • 1

克隆类型

  • 63
  • 20
多克隆

标记

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  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This RPA1 antibody is un-conjugated

应用范围

  • 71
  • 29
  • 22
  • 20
  • 20
  • 15
  • 14
  • 14
  • 13
  • 9
  • 3
  • 3
  • 1
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  • 1
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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
  • 抗原表位

    • 16
    • 9
    • 7
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 350-400

    原理

    Rabbit anti-RPA70 Antibody, Affinity Purified

    预测反应

    Orangutan

    纯化方法

    Affinity Purified

    免疫原

    between AA 350 and 400

    亚型

    IgG
  • 应用备注

    IHC: 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

    IP: 2 - 10 μg/mg lysate

    WB: 1:2,000 - 1:10,000

    限制

    仅限研究用
  • 浓度

    1000 μg/mL

    缓冲液

    Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    有效期

    12 months
  • 抗原

    RPA1 (Replication Protein A1, 70kDa (RPA1))

    别名

    RPA70

    背景

    Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA1, the 70 kDa subunit, is an ssDNA binding protein that has been shown to be phosphorylated by ATM and ATR in response to DNA damage. In addition to a role in MMR, RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication.

    基因ID

    6117

    NCBI登录号

    NP_002936

    UniProt

    P27694

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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