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Recombinant PCNA 抗体 (AA 225-261)

This anti-PCNA antibody is a 兔 单克隆 antibody detecting PCNA in WB, IHC, IF (p) 和 ICC. Suitable for 人.
产品编号 ABIN7449543
发货至: 中国

Quick Overview for Recombinant PCNA 抗体 (AA 225-261) (ABIN7449543)

抗原

See all PCNA 抗体
PCNA (Proliferating Cell Nuclear Antigen (PCNA))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This PCNA antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC)

克隆位点

BLR075G
  • 抗原表位

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    AA 225-261

    原理

    Rabbit anti-PCNA Recombinant Monoclonal Antibody [BLR075G]

    免疫原

    between AA 225 and 261 (C-term)

    亚型

    IgG
  • 应用备注

    IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

    WB: 17:40:00

    限制

    仅限研究用
  • 浓度

    10 μg/mL

    缓冲液

    Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    有效期

    12 months
  • 抗原

    PCNA (Proliferating Cell Nuclear Antigen (PCNA))

    别名

    PCNA

    背景

    Background: PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

    基因ID

    5111

    NCBI登录号

    NP_002583

    UniProt

    P12004

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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