Recombinant PCNA 抗体 (AA 225-261)
Quick Overview for Recombinant PCNA 抗体 (AA 225-261) (ABIN7449543)
抗原
See all PCNA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
 - AA 225-261
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原理
 - Rabbit anti-PCNA Recombinant Monoclonal Antibody [BLR075G]
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免疫原
 - between AA 225 and 261 (C-term)
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亚型
 - IgG
 
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应用备注
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IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
WB: 17:40:00
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限制
 - 仅限研究用
 
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浓度
 - 10 μg/mL
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缓冲液
 - Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C
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有效期
 - 12 months
 
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- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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别名
 - PCNA
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背景
 - Background: PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
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基因ID
 - 5111
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NCBI登录号
 - NP_002583
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UniProt
 - P12004
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途径
 - Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
 
抗原
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