IGF1 抗体 (Secreted)
Quick Overview for IGF1 抗体 (Secreted) (ABIN7237701)
抗原
See all IGF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 74-85, Secreted
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原理
- A Rabbit Polyclonal antibody to IGF1
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特异性
- Secreted
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 （详细）
- The antibody is specific for IGF1, it does not crossreact with IGF2.
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预测反应
- Mouse - identical, Human - 11 out of 12 amino acid residues identical
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产品特性
- Secreted
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)NKPTGYGSSIRR, corresponding to amino acid residues 74 - 85 of rat IGF1
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亚型
- IgG
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anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibody
IGF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibodyIGF1 适用: 人 WB, ICC, IF 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) antibodyIGF1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-153) antibodyIGF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (Internal Region) antibodyVerified IGF1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 115-144) antibodyIGF1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 49-100) antibodyIGF1 适用: 人, 小鼠, 大鼠, 犬 WB, IP, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 33-102) antibodyIGF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 115-144), (C-Term) antibodyIGF1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB33711 unconjugated
anti-Insulin-Like Growth Factor 1 (IGF1) (AA 50-150) antibodyIGF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235814)
Blocking Peptide: (ABIN7235814)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF1
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背景
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Insulin Like Growth Factor 1, Insulin-Like Growth Factor I, IGF-I, Somatomedin-C,IGF-1 originally called somatomedin C is a 70-amino acid hormone with homology to IGF-2 and proinsulin. IGF-1 acts as an endocrine, paracrine, and autocrine hormone and is known as a mediator of prenatal and postnatal growth. IGF-1 is mainly synthesized in the liver and regulated by growth hormones (GH) in peripheral tissues such as muscle, cartilage, bone, kidney, nerves, skin, lungs, and the liver itself. The synthesized IGF-1 binds to IGF-1 binding proteins (IGFBPs) with high affinity, IGFBPs regulates the interactions of IGF-1 and its receptor (IGF-1R)1. Binding of IGF-1 to his receptor results in autophosphorylation of the receptor leading to activation of the insulin receptor substrate 1 (IRS1) protein. The activated receptor contains several phosphotyrosine sites that serve as docking sites for many signaling proteins. The association of these proteins together start a complex signaling pathway including the canonical PI3-kinase and mitogen-activated protein (MAP) kinase pathways2.The IGF-1 signaling pathway is a major regulator of the aging process, from rodents to humans3. IGF-1 deficiency follows an autosomal mode of transmission and different mutations can lead to either IGF-1 deletion or low levels of circulation IGF-1. Studies demonstrate IGF-1 deficit can lead to deregulated lipid metabolism, cardiovascular disease (CVD), diabetes, and altered metabolic profile of diabetic patients4.
Alternative names: Insulin Like Growth Factor 1, Insulin-Like Growth Factor I, IGF-I, Somatomedin-C -
基因ID
- 24482
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NCBI登录号
- NM_000618
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UniProt
- P08025
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
抗原
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