SNCA 抗体 (AA 117-131)
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- 抗原 See all SNCA 抗体
- SNCA (Synuclein, alpha (SNCA))
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抗原表位
- AA 117-131
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SNCA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- This antibody has been shown to react strongly with human brain-derived α-synuclein. The antibody also reacts with rat brain-derived α-synuclein but reactivity appears substantially less than with human.
- 交叉反应 (详细)
- Does not cross-react with mouse α-synuclein.
- 产品特性
- NACP| Non-A β component of senile plaques,alpha-Synuclein polyclonal antibody
- 纯化方法
- Purified by caprylic acid and ammonium sulphate precipitation.,Purified from rabbit serum. Vial contains an immunoglobulin preparation, partially purified by chromatography from whole serum, suspended in phosphate-buffered saline (pH 7.4) containing 0.01M sodium azide.
- 免疫原
- Synthetic peptide corresponding to [Cys] + aa 117-131 of human alpha-synuclein conjugated to KLH. The sequence is conserved in rat alpha-synuclein except for two substitutions, namely Asp121Ser and Asn122Ser.
- Top Product
- Discover our top product SNCA Primary Antibody
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- 应用备注
- Western blot (1:1,000-1:20,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.,Western blot: The antibody has been characterized by single dimension SDS-PAGE using recombinant α- and β-synucleins as well as rat, mouse and human brain lysates with and without peptide blockade. Studies with the recombinant proteins demonstrated the absolute specificity of the antibody for α-synuclein. The antibody gives a strong band at a relative molecular weight of approximately 15 kDa with human brain lysate which is wholly abolished by preincubation with the immunizing peptide (Prod. No. BML-SP9340).Immunohistochemistry: The antibody has been tested on de-waxed tissue sections of human brain fixed in neutral buffered formaldehyde. A standard protocol should be followed.
- 说明
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For Blocking peptide see Prod. No. BML-SP9340.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Liquid. In PBS, pH 7.4, containing 10 mM sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 °C.
- 储存条件
- -20 °C
- 储存方法
- Store unopened vial at -20°C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality 'antiserum-grade' plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
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- 抗原
- SNCA (Synuclein, alpha (SNCA))
- 别名
- alpha-Synuclein (SNCA 产品)
- 别名
- snca antibody, MGC64356 antibody, LOC619283 antibody, NACP antibody, alphaSYN antibody, PARK1 antibody, PARK4 antibody, PD1 antibody, synuclein alpha L homeolog antibody, alpha-synuclein antibody, synuclein alpha antibody, synuclein, alpha antibody, snca.L antibody, LOC619283 antibody, SNCA antibody, Snca antibody
- UniProt
- P37840
- 途径
- Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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