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TBP 抗体 (AA 120-200)

This 兔 多克隆 anti-TBP antibody specifically detects TBP in WB, ELISA, IF 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7217282
发货至: 中国
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Quick Overview for TBP 抗体 (AA 120-200) (ABIN7217282)

抗原

See all TBP 抗体
TBP (TATA Box Binding Protein (TBP))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This TBP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 120-200

    原理

    TFIID Polyclonal Antibody

    特异性

    TFIID Polyclonal Antibody detects endogenous levels of TFIID protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    免疫原

    Synthesized peptide derived from the Internal region of human TFIID at AA range: 120-200

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

    说明

    Primary Antibody

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原

    TBP (TATA Box Binding Protein (TBP))

    别名

    TFIID

    背景

    Rabbit Anti-TFIID Polyclonal Antibody,TBP, GTF2D1, TF2D, TFIID, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit,Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. TATA-box-binding protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.,TATA-box-binding protein

    分子量

    observerd band 38kDa

    基因ID

    6908

    UniProt

    P20226

    途径

    WNT signaling
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