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NF-kB p65 抗体 (pSer536)

This 兔 多克隆 anti-NF-kB p65 antibody specifically detects NF-kB p65 in WB, ELISA, IP, IF 和 IHC (p). The antibody is reactive with 人, 小鼠, 大鼠 和 猴 samples.
产品编号 ABIN7207207
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for NF-kB p65 抗体 (pSer536) (ABIN7207207)

抗原

See all NF-kB p65 (NFkBP65) 抗体
NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

适用

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人, 小鼠, 大鼠, 猴

宿主

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克隆类型

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多克隆

标记

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This NF-kB p65 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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    pSer536

    原理

    NFκB-p65 (phospho Ser536) Polyclonal Antibody

    特异性

    Phospho-NFκB-p65 (S536) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S536.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    免疫原

    Synthesized peptide derived from human NFkappaB-p65 Phospho-Ser536

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), IP (2-5 μg/mg lysate), ELISA (1:10000). Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原

    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

    别名

    NFkappaB-p65

    背景

    Rabbit Anti-NFκB-p65 (phospho Ser536) Polyclonal Antibody,RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for RELA.,Transcription factor p65

    基因ID

    5970

    UniProt

    Q04206

    途径

    NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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